Understanding PCR

• Author : Sarah Maddocks,Rowena Jenkins
• Publisher : Academic Press
• Release Date : 2016-10-27
• ISBN : 9780128026977

Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. Includes resources for reagents Explains basic laboratory preparation Provides straightforward experimental protocols Incorporates fundamental analytical techniques Contains a troubleshooting

• Author : MCPHERSON.,S. G. Møller
• Publisher : Garland Science
• Release Date : 2000
• ISBN : 9781859960172

A thoroughly updated version of the successful first edition, with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections.

• Author : Mike McPherson,Simon Møller
• Publisher : Garland Science
• Release Date : 2007-01-25
• ISBN : 9780203002674

A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.

• Author : Teri Shors
• Publisher : Jones & Bartlett Publishers
• Release Date : 2011-11-08
• ISBN : 9781449677541

The ideal text for undergraduate students majoring in biology, microbiology, medical technology, or pre-med, the Second Edition of Understanding Viruses provides a balanced approach to this fascinating discipline, combining the molecular, clinical, and historical aspects of virology. Updated throughout to keep pace with this fast-paced field, the text provides a strong, comprehensive introduction to human viral diseases. New material on molecular virology as well as new virus families presented coupled with chapters on viral diseases of animals; the history of

• Author : Michael A. Innis,David H. Gelfand,John J. Sninsky
• Publisher : Elsevier
• Release Date : 1995-07-06
• ISBN : 9780080538549

PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR. The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to

• Author : Patricia Hernandez-Rodriguez
• Publisher : BoD – Books on Demand
• Release Date : 2012-05-30
• ISBN : 9789535106128

This book is intended to present current concepts in molecular biology with the emphasis on the application to animal, plant and human pathology, in various aspects such as etiology, diagnosis, prognosis, treatment and prevention of diseases as well as the use of these methodologies in understanding the pathophysiology of various diseases that affect living beings.

• Author : John M. S. Bartlett,David Stirling
• Publisher : Springer Science & Business Media
• Release Date : 2008-02-03
• ISBN : 9781592593842

In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of

• Author : Francois Ferre
• Publisher : Springer Science & Business Media
• Release Date : 2012-12-06
• ISBN : 9781461241645

Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a

• Author : Elizabeth van Pelt-Verkuil,Alex van Belkum,John P. Hays
• Publisher : Springer Science & Business Media
• Release Date : 2008-03-14
• ISBN : 9781402062414

Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).

• Author : Y. M. Dennis Lo
• Publisher : Springer Science & Business Media
• Release Date : 1998
• ISBN : 9781592596003

Clinical Applications of PCR offers an unprecedented collection of core PCR techniques for the study and diagnosis of human diseases. Cutting-edge and essential for today's diagnostic laboratories, these techniques heavily utilize nonisotopic, solution phase, and in situ amplification methods. A significant number of chapters describe applications exploiting the exquisite sensitivity of PCR in the detection of rare or single cells, as in identifying fetal cells circulating in maternal blood, preimplantation embryo diagnosis, or detecting circulating cancer cells. The methods described

• Author : John P. Vanden Heuvel
• Publisher : CRC Press
• Release Date : 2019-05-07
• ISBN : 1439805806

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques

• Author : Joseph W. St. Geme, III
• Publisher : Springer
• Release Date : 2016-08-26
• ISBN : 9783319437293

This book describes the growing body of information on the epidemiology, clinical manifestations, transmission, pathogenesis, diagnosis, and treatment of Kingella kingae infections in young children. In addition, it covers experimental methods that have been developed to study the microbiology, genetics, and virulence factors of K. kingae, information that provides the foundation for new approaches to treatment and prevention of K. kingae disease. With this content in mind, excerpts from the book will be of relevance for clinicians who care for

• Author : John P. Vanden Heuvel
• Publisher : CRC Press
• Release Date : 2019-05-07
• ISBN : 9780429526091

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques

• Author : Konrad Sachse,Joachim Frey
• Publisher : Springer Science & Business Media
• Release Date : 2003
• ISBN : 9781588290496

Hands-on laboratory experts present a set of "classic" PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. These proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and

• Author : Genetic Alliance,New York-Mid-Atlantic Consortium for Genetic and Newborn Screening Services
• Publisher : Lulu.com
• Release Date : 2009
• ISBN : 9780982162217

The purpose of this manual is to provide an educational genetics resource for individuals, families, and health professionals in the New York - Mid-Atlantic region and increase awareness of specialty care in genetics. The manual begins with a basic introduction to genetics concepts, followed by a description of the different types and applications of genetic tests. It also provides information about diagnosis of genetic disease, family history, newborn screening, and genetic counseling. Resources are included to assist in patient care,